Human Anti-SIRPA Recombinant Antibody (PABJ-0078)
CAT#: PABJ-0078
This product is a Human antibody that recognizes Human SIRPA. This antibody binds to SIRPα at regions distal from the CD47 binding site.
Tested Data
Gene Expression
ELISA
Figure 1 Human Anti-SIRPA Antibody (PABJ-0078) in ELISA
ELISA analysis of PABJ-0078 was performed by coating with Recombinant Human SIRPA.
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Human Anti-SIRPA Antibody (PABJ-0078) in WB
Western blot analysis of PABJ-0078 was performed by loading Recombinant Human SIRPA.
PABJ-0078 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane1: Reducing antigen (0.1 μg)
Lane2: Reducing antigen (0.3 μg)
Lane3: Reducing antigen (0.6 μg)
DB
Figure 3 Human Anti-SIRPA Antibody (PABJ-0078) in DB
Dot blot analysis of PABJ-0078 was performed by loading Recombinant Human SIRPA.
PABJ-0078 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human SIRPA
- Species Reactivity
- Human, Monkey
- Applications
- ELISA
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- SIRPA; Inhibitory Receptor SHPS-1; Signal-Regulatory Protein Alpha-3; CD172A; CD172 Antigen-Like Family Member A; P84; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-3; MFR; SHPS1; CD172a Antigen; Signal-Regulatory Protein Alpha-2; Macrophage
- Gene ID
- 140885
- UniProt ID
- P78324
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
There are currently no Customer reviews or questions for PABJ-0078. Click the button above to contact us or submit your feedback about this product.
Robert Chang30.Sep,21
Working well in WB
Great performance overall. The antibody worked well in Western blotting, giving us clear bands with minimal background. My only minor issue was with the initial dilution optimization, but customer service was very helpful in guiding us.
Maria Fernandez23.Sep,21
Good for immunoprecipitation experiments
The greatest SIRPA antibody we have yet to utilize. In our immunoprecipitation studies, it has been quite helpful in delivering clear and powerful pull-downs that have improved our comprehension of the SIRPA signaling pathway.
Q&As
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What is the specificity of Human Anti-SIRPA Recombinant Antibody (clone 218)?
A: The Signal Regulatory Protein Alpha (SIRPA) is the only protein that the Human Anti-SIRPA Recombinant Antibody (clone 218) is highly specific to and binds to with great affinity. Its assay-validated specificity guarantees accurate targeting in your experimental applications.
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Can this antibody be used for ELISA applications?
A: Yes, this antibody can be used in ELISA.
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How does the Human Anti-SIRPA Recombinant Antibody (clone 218) interact with macrophages, and does it influence phagocytosis?
A: By attaching itself to the CD47 receptor on macrophages, the Human Anti-SIRPA Recombinant Antibody (clone 218) blocks the "don't eat me" signal. This inhibition improves the phagocytosis of target cells, including cancer cells, by macrophages.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0078, RRID: AB_3111603)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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